Table 2 The sequences of forward and reverse primers and the size of intron of the representative 6 types IT markers
Marker no. | Forward primer (5’→3’) | Reverse primer (5’→3’) | Intron size of A genome | Intron size of B genome | Intron size of D genome | Intron size of V genome | Type |
|---|---|---|---|---|---|---|---|
CINAU687 | ACAGCTCATCATGCAGGACA | GTCACTGTCTTGAGCAAATGGA | 632 | 545 | 687 | 433 | TypeI |
CINAU648 | AGCCTCTCCTCCCTCAATCT | TGCTTCCACCTCAAATTGAACAT | 269 | 308 | 281 | 233 | TypeII |
CINAU737 | AAACGAGCTTTGCATGGAGG | CTTTGCATGTTGAGAAGGACAA | 339 | 146 | 340 | 119 | TypeIII |
CINAU665 | GCTCGGATGCAATTATTGTTGA | ATGGTCCTTCGCAGCTGTTA | 639 | 575 | 576 | 508 | TypeIV |
CINAU735 | TGAAGATCGTGTTCCTTCCTCT | CATGCTTTCTTCATCCCCTGG | 1583 | 1600 | 939 | 659 | TypeV |
CINAU646 | CATCGGTACTACGGGCGAT | TGCGGGTACTTCATCCTCAT | 298 | 304 | 307 | 445 | TypeVI |