Fig. 5

The relative expression level of Bm1645. a The qRT-PCR analysis of Bm1645 in TE-siRNAs-transfected BmN cells. The expression was downregulated when the BmN cells were transfected with TE-siRNAs. A negative control was used as contrast. b The transcription level of Bm1645 affected by BmAGO2 knockdown in BmN cells. We used the RNA interference method to downregulate the expression of BmAGO2. The result shows the transcriptional level of Bm1645 was upregulated 1.66 times, as assessed by qRT-PCR. Each histogram bar represents the mean relative expression of the indicated transcripts, with at least three replicates for each bar. The asterisks (*, ** or ***) indicate the significant differences (P < 0.05, P < 0.01 or P < 0.001, respectively) compared with the relevant control with a two-tailed t-test