Fig. 4

MSA of selected conserved regions of DGAT1s from algae and examples from other representative taxa. The black horizontal arrows represent A. thaliana DGAT1 and the predicted transmembrane domains, respectively. Motifs 1–7 correspond to the conserved regions defined by Cao [29]. The sequences shown cluster either into the DGAT1 clade or the DGAT1-like clade. The alignment corresponds to motif 6 and parts of motifs 5 and 7, where the main differences between the sequences within the DGAT1-like clade and the rest of the DGAT1s in the Archaeplasida supergroup lie. The level of conservation increases from light gray to dark gray. The dashed boxes depict the most remarkable dissimilarities between the DGAT1-like clade and their DGAT1 clade homologs from the Archaeplastida group. Sequences are indicated with their internal codes, first letter of the genus and first three letters of the species (see Additional file 1 for details). IDs correspond to Uniprot IDs when available or to specific genome IDs when the sequence was not found in Uniprot. Taxonomic classification at higher ranks is shown on the left