From: Transcriptomic buffering of cryptic genetic variation contributes to meningococcal virulence
α522 | MC58 | |
---|---|---|
Genome characteristics | ||
 GenBank accession number | FR845693 to FR845718 | AE002098 |
 No. of contigs in final assembly | 21 | 1 |
 Average single base coverage | 79-fold | 8.4-fold |
 Genome size (bp) | ≥2,074,170 | 2,272,360 |
 GC content (%) | 51.78% | 51.53% |
 Predicted number of coding sequences | ≥1985 | 2063 |
 Reference | This work | Tettelin et al. (2000) [123] |
Molecular epidemiology | ||
 Source | Carrier | Patient |
 Country and year of isolation | Germany 2000 | United Kingdom 1983 |
 Sequence type | ST-35 | ST-74 |
 Clonal complex (CC) | ST-35 | ST-32 |
 Phylogenetic clade (PC) (a) | PC32/269 | PC32/269 |
 Frequency of CC in carriers(b) | 5.47% | 4.99% |
 Disease/carriage ratio(c) | 0.5 | 3.5 |
 Reference | Claus et al. (2005) [100] | McGuiness et al. (1991) [101] |
Phenotypic characterization | ||
 Serum resistance (%)(d) | 117.7 ± 23.8 | 116.3 ± 15.7 |
 Adhesion to epithelial cells(e) | ||
  FaDu cells (%) | 10.7 ± 7.4 | 14.5 ± 8.5 |
  Detroit562 cells (%) | 8.9 ± 1.8 | 17.1 ± 5.3 |
 Invasion of epithelial cells(e) | ||
  FaDu cells (%) | 0.0008 ± 0.0001 | 0.0020 ± 0.0011 |
  Detroit562 cells (%) | 0.0011 ± 0.0004 | 0.0016 ± 0.0007 |
In vitro logarithmic growth rates(f) | ||
  Rich medium (PPM+) (1/h) | 0.46 ± 0.01 | 0.47 ± 0.01 |
  Minimal medium (MMM) (1/h) | 0.06 ± 0.01 | 0.52 ± 0.02 |
Ex vivo growth rates(g) | ||
  Saliva (1/min) | −0.041 ± 0.003 | −0.043 ± 0.003 |
  Blood (1/min) | 0.027 ± 0.004 | 0.023 ± 0.002 |
  CSF (1/min) | 0.010 ± 0.002 | 0.018 ± 0.003 |