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Table 1 Strains used for ex vivo transcriptome comparisons

From: Transcriptomic buffering of cryptic genetic variation contributes to meningococcal virulence

  α522 MC58
Genome characteristics
 GenBank accession number FR845693 to FR845718 AE002098
 No. of contigs in final assembly 21 1
 Average single base coverage 79-fold 8.4-fold
 Genome size (bp) ≥2,074,170 2,272,360
 GC content (%) 51.78% 51.53%
 Predicted number of coding sequences ≥1985 2063
 Reference This work Tettelin et al. (2000) [123]
Molecular epidemiology
 Source Carrier Patient
 Country and year of isolation Germany 2000 United Kingdom 1983
 Sequence type ST-35 ST-74
 Clonal complex (CC) ST-35 ST-32
 Phylogenetic clade (PC) (a) PC32/269 PC32/269
 Frequency of CC in carriers(b) 5.47% 4.99%
 Disease/carriage ratio(c) 0.5 3.5
 Reference Claus et al. (2005) [100] McGuiness et al. (1991) [101]
Phenotypic characterization
 Serum resistance (%)(d) 117.7 ± 23.8 116.3 ± 15.7
 Adhesion to epithelial cells(e)
  FaDu cells (%) 10.7 ± 7.4 14.5 ± 8.5
  Detroit562 cells (%) 8.9 ± 1.8 17.1 ± 5.3
 Invasion of epithelial cells(e)
  FaDu cells (%) 0.0008 ± 0.0001 0.0020 ± 0.0011
  Detroit562 cells (%) 0.0011 ± 0.0004 0.0016 ± 0.0007
In vitro logarithmic growth rates(f)
  Rich medium (PPM+) (1/h) 0.46 ± 0.01 0.47 ± 0.01
  Minimal medium (MMM) (1/h) 0.06 ± 0.01 0.52 ± 0.02
Ex vivo growth rates(g)
  Saliva (1/min) −0.041 ± 0.003 −0.043 ± 0.003
  Blood (1/min) 0.027 ± 0.004 0.023 ± 0.002
  CSF (1/min) 0.010 ± 0.002 0.018 ± 0.003
  1. (a) According to ref. [27, 28]
  2. (b) According to ref. [100]
  3. (c) According to ref. [2]
  4. (d) Ratio in percent of viable bacteria after incubation for 30 min in the presence of 10% human serum and viable bacteria incubated without serum. Given are the average and standard deviation from four independent experiments with pooled human serum
  5. (e) Ratio in percent of adherent and invasive bacteria, respectively, to total bacteria. Given are the average and standard deviation from at least four independent experiments
  6. (f) Given are the mean and standard deviation of the logarithmic growth rate k according to log(OD 600 (t)/OD 600 (0)) = kt for t [1 h, 4 h] as depicted in Fig. 5 using linear regression (R 2 in vitro  = 0.96 ± 0.07, p in vitro  = 0.013 ± 0.026)
  7. (g) Given are the mean and standard deviation of the growth rate k according to log(N(t)/N(0)) = kt for t [0 min, 120 min] as depicted in Fig. 5 using linear regression (R 2 ex vivo = 0.95 ± 0.05, p ex vivo = 0.018 ± 0.016)