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Table 1 Strains used for ex vivo transcriptome comparisons

From: Transcriptomic buffering of cryptic genetic variation contributes to meningococcal virulence

 

α522

MC58

Genome characteristics

 GenBank accession number

FR845693 to FR845718

AE002098

 No. of contigs in final assembly

21

1

 Average single base coverage

79-fold

8.4-fold

 Genome size (bp)

≥2,074,170

2,272,360

 GC content (%)

51.78%

51.53%

 Predicted number of coding sequences

≥1985

2063

 Reference

This work

Tettelin et al. (2000) [123]

Molecular epidemiology

 Source

Carrier

Patient

 Country and year of isolation

Germany 2000

United Kingdom 1983

 Sequence type

ST-35

ST-74

 Clonal complex (CC)

ST-35

ST-32

 Phylogenetic clade (PC) (a)

PC32/269

PC32/269

 Frequency of CC in carriers(b)

5.47%

4.99%

 Disease/carriage ratio(c)

0.5

3.5

 Reference

Claus et al. (2005) [100]

McGuiness et al. (1991) [101]

Phenotypic characterization

 Serum resistance (%)(d)

117.7 ± 23.8

116.3 ± 15.7

 Adhesion to epithelial cells(e)

  FaDu cells (%)

10.7 ± 7.4

14.5 ± 8.5

  Detroit562 cells (%)

8.9 ± 1.8

17.1 ± 5.3

 Invasion of epithelial cells(e)

  FaDu cells (%)

0.0008 ± 0.0001

0.0020 ± 0.0011

  Detroit562 cells (%)

0.0011 ± 0.0004

0.0016 ± 0.0007

In vitro logarithmic growth rates(f)

  Rich medium (PPM+) (1/h)

0.46 ± 0.01

0.47 ± 0.01

  Minimal medium (MMM) (1/h)

0.06 ± 0.01

0.52 ± 0.02

Ex vivo growth rates(g)

  Saliva (1/min)

−0.041 ± 0.003

−0.043 ± 0.003

  Blood (1/min)

0.027 ± 0.004

0.023 ± 0.002

  CSF (1/min)

0.010 ± 0.002

0.018 ± 0.003

  1. (a) According to ref. [27, 28]
  2. (b) According to ref. [100]
  3. (c) According to ref. [2]
  4. (d) Ratio in percent of viable bacteria after incubation for 30 min in the presence of 10% human serum and viable bacteria incubated without serum. Given are the average and standard deviation from four independent experiments with pooled human serum
  5. (e) Ratio in percent of adherent and invasive bacteria, respectively, to total bacteria. Given are the average and standard deviation from at least four independent experiments
  6. (f) Given are the mean and standard deviation of the logarithmic growth rate k according to log(OD 600 (t)/OD 600 (0)) = kt for t ∈ [1 h, 4 h] as depicted in Fig. 5 using linear regression (R 2 in vitro  = 0.96 ± 0.07, p in vitro  = 0.013 ± 0.026)
  7. (g) Given are the mean and standard deviation of the growth rate k according to log(N(t)/N(0)) = kt for t ∈ [0 min, 120 min] as depicted in Fig. 5 using linear regression (R 2 ex vivo = 0.95 ± 0.05, p ex vivo = 0.018 ± 0.016)