Transcriptomic buffering of cryptic genetic variation contributes to meningococcal virulence

BMC Genomics

Table 4 Inferred reaction activity differences between MC58 and α522 in human blood based on gene cross-strain expression differences

Reaction	Chemical Equation	Enzyme(s)	Ratio^(a)
R030	AKG + NADPH + NH₃ = GLU + NADP	Glutamate dehydrogenase	−14.85
R031	ATP + GLU + NH₃ = ADP + GLN	Glutamine synthetase	−3.03
R047	AcCoA + H2S + SER = ACE + CYS + CoA	Serine acetyltransferase + cysteine synthase	−2.32
R001	AcCoA + OXA = CIT + CoA	Citrate-synthase	13.86
R002	NADP + CIT = CO₂ + NADPH + AKG	Aconitase	13.86
R015	CO₂ + PEP = OXA	Phosphoenolpyruvate carboxylase	11.90
R052	3PG + GLU + NAD = AKG + NADH + SER	Serine synthesis: 3PG dehydrogenase + pserine aminotransferase + pserine phosphatase (SerA+ SerB + SerC)	7.63
R053	SER = GLY	Serine hydroxymethyltransferase	7.45
R056	AcCoA + GLU + IVA + NAD = AKG + CO₂ + CoA + LEU + NADH	Leucine synthesis: isopropylmatate synthase + isopropylmalate dehydratase + isopropylmalate dehydrogenase + aminotransferase (LeuA + LeuB + LeuC + LeuD)	6.78
R061	CHOR = PRE	Chorismate mutase	12.58
R062	GLU + PRE = AKG + CO₂ + PHE	Aminotransferase + phenyalanine synthesis	12.58
R063	GLU + NAD + PRE = AKG + CO₂ + NADH + TYR	Aminotransferase + tyrosine synthesis	12.58
R117	ACA + NADH = ETH + NAD	Alcohol dehydrogenase	14.74
R118	ACE + NADH = ACA + NAD	Aldehyde dehydrogenase	14.74
R155	ACE = ACE_ext + H_ext	Acetate transporter	11.50
R049	O₂ + THS = 2 SO₃	Thiosulfate reductase	0.61
R050	3 NADPH + SO₃ = H₂S + 3 NADP	Sulfite reductase	0.71
R132	H₂S = HS⁻_ext + H_ext	Sulfur transporter	0.45

^(a) Ratio of the reaction activity in MC58 divided by the reaction activity in α522. A ratio larger than one indicates that the reaction has a higher activity in MC58, and a negative ratio that the reaction occurs in opposite directions both strains

ISSN: 1471-2164