Fig. 1From: Quorum sensing of Streptococcus mutans is activated by Aggregatibacter actinomycetemcomitans and by the periodontal microbiomeThe alternative sigma-factor sigX of S. mutans is induced by A. act. when both species are co-cultured in a biofilm. a sigX expression determined by fluorescence intensity of S. mutans SMP sigX GFP, a gfp-reporter for sigX expression. b sigX expression determined by q-RT-PCR. The data were normalized to sigX expression in a S. mutans biofilm after 4 h. c Growth of S. mutans and A. act. in single- and dual-species biofilms determined by crystal violet staining. d Cell numbers of S. mutans and (e) A. act in single- and dual-species biofilms determined by q-PCR of the 16S rRNA gene. Mean and standard deviation of three independent experiments are shown for a–c, and of four independent experiments with two technical replicates for d–e Back to article page