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Fig. 6 | BMC Genomics

Fig. 6

From: Cross-talk of the biotrophic pathogen Claviceps purpurea and its host Secale cereale

Fig. 6

Expression of effector candidates. Expression levels of cp1105 (a + g), cp3095 (b), cp3096 (c), cp5492 (d), cp5493 (e) and cp8623 (f) in the wild type Cp20.1 during infection 3, 5 and 10 dpi as well as in axenic culture was determined by qRT-PCR and normalized to housekeeping genes. Expression of cp1105 (a), cp3095 (b), cp3096 (c), cp5492 (d), cp5493 (e) was strongly induced 3 and 5dpi and decreased 10 dpi as well as in axenic cultures. No clear expression pattern was detectable for cp8623 (f). Here, comparable expression rates were observed 5dpi in planta and in axenic culture. Expression of cp1105 was also determined in strains harboring a cp1105:mCherry construct from axenic culture (g). The experiments were done in two independent biological replicates, exemplary results from the second replicate are shown. Error bars indicate standard deviation from technical replicates

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