Perturbation of microbiota in one-day old broiler chickens with antibiotic for 24 hours negatively affects intestinal immune development

Table 1 Composition of the broiler diet

	broiler starter diet
Ingredient composition	(% inclusion)
Maize	35.03
Soybean meal	30
Wheat	27.6
Premix^a	2
Soybean oil	1.92
Palm oil	1
Chalk	0.7
Monocalcium phosphate	0.66
DL-methionine	0.26
Sodium bicarbonate	0.23
Lysine HCl	0.22
NaCl	0.19
L-threonine	0.05
Enzyme (NSP degrading ^b)	0.02
Enzyme (Phytase ^c)	0.01
Calculated composition	g/kg
Crude protein	209
Ether extract	56
Crude fibre	24.6
Ash	51.8
Starch (amylase)	388.6
Ca	7.8
P	5.2
Cl	1.7
Na	1.6
K	8.6
6-phytase (PU ^d/kg)	500
Digestible lysine	11.2
Digestible methionine	5.4
Digestible methionine + cysteine	8.3
Digestible threonine	6.9
Digestible tryptophan	2.2
Digestible isoleucine	7.7
Digestible valine	8.3
Digestible arginine	12.5
Digestible glycine + serine	16
Absorbable phosphorus	4.2
Analysed composition (g/kg)
ME (kcal/kg)	2900
Dry matter	873.4
Crude ash	50.6
Crude protein	201.1
Crude fat	60.5
Crude fibre	29.5
Starch	375.5
Calcium	7.7
Phosphorus	5.2

^aPremix supplied per kg of feed. Vitamin A, 12 500 (internation unit (IU); Vitamin D3 3500 IU; Vitamin Hy-D, 0.025 mg; Vitamin E, 100 IU; Vitamin K3, 4 mg; Vitamin B1 4 mg; Vitamin B2 9 mg; Pantothenic acid 20 mg; Niacinamide, 70 mg; Vitamin B6, 6 mg; Folate, 1.5 mg; Vitamin B12, 30 μg; Biotine 250, μg; Betaine, 150 mg; L-Carnitine, 30 mg; Fe, 50.0 mg; I, 2.0 mg; Cu, 14.0 mg; Mn, 55 mg; Zn, 100 mg; Se, 0.3 mg
^bHostazyme X®
^cPhyzyme® XP 5000 L
^dPU, phytase units

ISSN: 1471-2164