Fig. 4

Systematic analysis of dysregulated pathways in INTS12 depleted cells. a Cleveland’s plot showing the GSEA results of representative D-siRNA A analysis. Only pathways reproducibly dysregulated in both D-siRNA treatments and not dysregulated by non-specific D-siRNA control treatment are included. The shade of colour indicates statistical significance of enrichment after multiple hypotheses testing correction. The size of dot reflects the number of statistically significant differentially expressed gene. The location of dot reflects enrichment score in pathway analysis. b Enrichment plots of cytosolic tRNA aminoacylation and PERK regulated gene expression pathways in D-siRNA A analysis. The FDR values were 0.0004 and 0.002 while normalized enrichment scores were −2.05 and −1.95 for tRNA aminoacylation and PERK regulated gene expression respectively. c Heatmaps of genes belonging to tRNA aminoacylation and PERK regulated gene expression pathways. Samples were clustered by unsupervised hierarchical clustering and resulted in clustering of three biological replicate samples of each of the four conditions: un-transfected cells (UT), cells transfected with scrambled D-siRNA negative control (NC), cells transfected with anti-INTS12 D-siRNA A (A) and cells transfected with anti-INTS12 D-siRNA C (C). Green and red colours on the Z-scale indicate lower and higher expression respectively