Fig. 7

Summary of INTS12 binding with HBEC epigenetic regulatory elements. a Percent of total INTS12 binding sites overlapping with HBEC-specific regulatory elements. Data from the first donor is shown as a representative of the two donors tested. Colour indicates the Z-score of the distance between the observed overlap and the mean of distribution of random overlap permutations. Negative Z-score implies that the observed overlap is less than expected by chance. Higher Z-score implies larger distance to the mean of distribution in a randomization test. Within P < 0.05 the maximum Z-score in random permutation walk is 8, 6, 4, 7 and 3 for H3K4me3, H3K36me3, H3K27me3, DNaseI, and CTCF respectively. The features most prominently localizing with INTS12 are H3K4me3 (Z-score = 348) and DNaseI (Z-score = 223) both marking transcriptionally active regions as well as CTCF (Z-score = 264). b Cross-correlations of INTS12 and HBEC specific regulatory elements ChIPseq signals on a genome-wide scale and in the promoter regions (TSS ± 3000 bp). Numbers represent Pearson’s correlations between ChIPseq signals of respective reference datasets. c Evolutionary conservation of INTS12 binding sites in vertebrates. The figure is showing the average phastcons score derived from multiple sequence alignment of vertebrate genomes, across the binding sites of INTS12 (red) and CTCF protein (blue)