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Table 4 Validation of microarray data for selected differentially expressed transcripts by qPCR

From: Analysis of hepatic transcript profile and plasma lipid profile in early lactating dairy cows fed grape seed and grape marc meal extract

  Mean FC P-value
Gene symbol Microarray qPCR Microarray qPCR
STMN1 2.57 1.37 0.015 0.269
ECT2 2.53 1.45 0.023 0.246
CENPA 2.49 1.16 0.006 0.514
CENPF 2.45 1.07 0.017 0.788
CKAP2 2.39 1.20 0.023 0.557
PRR11 2.32 1.93 0.036 0.181
KIF20A 2.21 1.66 0.017 0.088
BUB1B 2.20 1.22 0.004 0.528
RRM2 1.95 1.56 0.046 0.263
ESCO2 1.93 –1.02 0.032 0.905
SPC25 1.88 –1.19 0.034 0.207
CCNA2 1.87 –1.07 0.030 0.762
HMMR 1.87 –1.34 0.018 0.100
TUBB 1.85 1.38 0.013 0.040
GLCE –1.66 –1.2 0.036 0.195
MANF –1.59 –1.23 0.014 0.235
SAA4 –1.54 –1.31 0.043 0.057
SOCS3 –1.45 –1.14 0.042 0.522
PHLDA1 –1.43 –1.34 0.012 0.006
HYOU1 –1.39 –1.33 0.018 0.081
DNAJB11 –1.37 –1.23 0.026 0.170
BAG3 –1.37 1.07 0.032 0.674
UAP1 –1.34 1.05 0.005 0.674
CCNL1 –1.33 –1.26 0.009 0.067
CXCL14 –1.31 –1.16 0.036 0.459
  1. The microarray FC was calculated from the signal log ratios as follows: 2Signal log ratio if signal log ratio ≥ 0 and (−1) × 2–(Signal log ratio) if signal log ratio < 0. Signal log ratios were calculated from n = 6 microarrays per group. The qPCR FC was calculated analogously from normalized 2-ΔCt ratios. Normalized 2-ΔCt expression was calculated from n = 14 samples per group