Fig. 6

Klf9 represses transcription from synthetic promoters of Klf9 target genes, and Sp/Klf-like motifs are required for transcriptional repression by Klf9. We transfected HT22 [TR/TO-Klf9] cells with reporter constructs containing cloned DNA fragments corresponding to genomic regions with Klf9 peaks associated with the indicated genes (See Additional file 4: Figure S3 for genomic ranges). Forced expression of Klf9 repressed transcriptional activity from the Klf13, Klf16 and Limk1, but not from the Mapk11 promoter. Mutation of six Sp/Klf-like motifs in the Klf13 promoter abrogated Klf9-dependent repression. The asterisks indicate a statistically significant difference from control by Student’s two-sample t-test (p < .01)