Fig. 7

Klf9 target genes are dysregulated in Klf9 HT22 depleted cells and in Klf9 knockout mice, and depletion of Klf9 accelerates the cell cycle in HT22 cells. a Klf9 target genes are dysregulated in HT22 cells with Klf9 depleted by CRISPR/Cas9 genome editing. Asterisks indicate a statistically significant difference from the parent HT22 cell line (p < 0.05; ANOVA followed by Holm-Sidak post-hoc test). b Klf9 target genes are dysregulated in the hippocampus of postnatal day 7 Klf9-null mice. Asterisks indicate a statistically significant difference from wild-type mice by Student’s two-sample t-test (p < .05). c Cells with Klf9 depleted by CRISPR/Cas9 genome editing showed a higher percentage of cells in M phase (gray bars, lowercase letters) and a lower percentage in G1/G0 phase (black bars, uppercase letters). Means with the same letter are not significantly different (p < .05; ANOVA followed by Holm-Sidak post-hoc test). d The mRNA levels for two Klf9 target genes involved with cell cycle control are increased in Klf9 mutant HT22 cells. Means with the same letter are not significantly different (p < .05; ANOVA followed by Holm-Sidak post-hoc test)