Fig. 3From: High-throughput sequencing of pituitary and hypothalamic microRNA transcriptome associated with high rate of egg productionValidation of the miRNA expression profile by qRT-PCR. The relative expression levels of eight selected miRNAs were calculated according to the 2-ΔΔCt method using 5.8S rRNA as an internal reference RNA. Error bars represent the standard deviation. The x-axis indicates different miRNAs in the two tissues. *P < 0.05, **P <0.01, ***P < 0.001Back to article page