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Fig. 7 | BMC Genomics

Fig. 7

From: Genome-wide identification of conserved intronic non-coding sequences using a Bayesian segmentation approach

Fig. 7

RT-PCR of intronic putative functional elements (PFEs) showing their presence or absence in 24 hpf zebrafish cDNA pools. Each gene has between 1 and 7 PFEs. Exon lane contains an exonic region, spanning an intron, of the gene of interest. Intron lane represents a randomly selected intronic region that was not identified as a PFE. Primers were designed to amplify products with sizes ranging 57-274 bp. The ladder bands shown are 100, 200 and 300 bp. The gels with the two bands of the ladder showing are the 100 and 200 bp bands. The panel insert is a cDNA control. β-actin (exonic spanning an intron) and RNA (RNA used as a template) lanes demonstrate there is no genomic contamination. No template lane rules out contamination of other PCR reagents

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