Fig. 5

GEX1A treatment induced RD29A-LUC expression and stomatal aperture closure, caused relocation of SR45 proteins. a, One-week-old RD29A-LUC transgenic seedlings were treated with DMSO, 100 μM ABA or 5 μM GEX1A for 6 h, then sprayed with D-luciferase and observed by CCD camera. b, Relative bioluminescence intensities of RD29A-LUC seedlings in each treatment. c, Leaves of 3–4-week-old Arabidopsis plants were treated in opening solution for approximately 2.5 h and then transferred into opening solution with 20 μM GEX1A for 4 h. DMSO and ABA were used as negative and positive controls, respectively. d, Boxplot comparison of stomatal aperture in Arabidopsis leaves after the indicated treatments, three replicates and 150 stomata were measured. e, One-week-old 35S::SR45:GFP transgenic seedlings were treated with DMSO (left) or 5 μM GEX1A (right) for 24 h. Left-upper, GFP signal in the elongation zone of a 35S::SR45:GFP root in control conditions. Left-bottom, close-up view of nuclei of elongation zone cells from DMSO-treated 35S:SR45:GFP transgenic plants. Right-upper, GFP signal in the elongation zone of a 35S::SR45:GFP root in GEX1A treatment. Right-bottom, close-up view of nuclei of elongation zone cells from 5 μM GEX1A-treated 35S::SR45:GFP transgenic plants, nuclear speckles formed in the nuclei