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Table 6 Validation of MBD-Seq Differential Expression

From: Male fertility status is associated with DNA methylation signatures in sperm and transcriptomic profiles of bovine preimplantation embryos

CpG DMR on CHR12
(% methylated)
DMR on CHR19
(% methylated)
High
(n = 28)
Low
(n = 30)
High
(n = 31)
Low
(n = 39)
1 80.8 11.1 0 0
2 90.0 24.0 0 0
3 95.5 9.1 17.2 14.7
4 90.0 25.0 16.2 8.1
5 81.8 25.9 6.9 3.1
6 47.8 3.5 11.1 24.3
7 95.0 18.8 26.7 38.9
8 80.8 13.8 38.5 38.5
9 90.0 5.3 9.7 23.9
10 80.8 26.9 9.7 34.3
11 90.0 35.0 10.0 37.8
12 80.8 10.5 21.4 46.2
13 81.5 37.0 22.6 41.0
14 90.0 70.0 26.7 43.6
15    30.0 43.6
16    23.3 65.8
17    34.5 55.6
18    12.9 32.4
19    3.8 28.6
20    22.6 8.1
21    32.3 40.0
22    43.3 51.3
23    37.9 44.4
Total: 82.1%* 20.6% 20.8% 34.0%*
  1. *Denotes a statistically significant difference (P < 0.0001) between total methylated CpG sites for a given DMR between high and low fertility spermatozoa. n = the number of clones sequenced
  2. The DMRs identified on chromosome 12 and chromosome 19 were assessed for differential levels of methylation by bisulfite conversion followed by Sanger sequencing