Table 2 Summary of sequences analysis and RNA-Seq data

Raw reads

172,380,454

168,368,500

Clean reads

165,289,336

161,562,282

Clean bases

24.79G

24.24G

Error rate (%)

0.01

0.01

Q20 (%)

97.97

98.01

Q30 (%)

94.95

94.99

GC content (%)

48.03

47.91

Total mapped

111,825,953 (67.63%)

112,860,042 (69.89%)

Multiple mapped

2,211,063 (1.33%)

2,111,248 (1.33%)

Uniquely mapped

109,614,890 (66.30%)

110,748,794 (68.57%)

Reads map to ‘ + ’

54,945,243 (33.24%)

55,565,180 (34.40%)

Reads map to ‘-’

54,669,647 (33.07%)

55,183,614 (34.16%)

Non-splice reads

63,472,079 (38.40%)

65,056,741 (40.27%)

Splice reads

46,142,811 (27.90%)

45,692,053 (28,29%)