Fig. 3

Dcr2 only interacts with the CCC in the nucleus. a Dcr2 is present in the nucleus. WB of refined nuclear (NE) and cytoplasmic extracts (CE) reveals a nuclear pool of Dcr2 (top). b Endogenous Dcr2 co-IPs the CCC and R2D2 from refined NEs (top). No interaction between Dcr2 and the CCC is observed in CE (bottom). Antibodies used for IP are shown above. Antibodies used for WB are listed left. ‘Beads’ and ‘α-Myc’ are negative controls. A lighter exposure of the R2D2 WB is shown at the bottom of the top NE group