Table 1 Illumina NextSeq500 read processing and mapping results from RNA-seq mountain pine beetle libraries
Sample ID | Sample description | Biological replicate | Index 1 | Index 2 | Total read pairs | Processed read pairs | Aligned | Unique counts |
|---|---|---|---|---|---|---|---|---|
MFF1 | Adult fed female midgut/fat body | 1 | TCCGGAGA | AGGCTATA | 32908461 | 32869809 | 25376605 | 20575844 |
MFF2 | Adult fed female midgut/fat body | 2 | TCCGGAGA | GCCTCTAT | 33943439 | 33904830 | 22563448 | 18078917 |
MFF3 | Adult fed female midgut/fat body | 3 | TCCGGAGA | AGGATAGG | 24984038 | 24959212 | 18972883 | 15336322 |
MFF4 | Adult fed female midgut/fat body | 4 | TCCGGAGA | TCAGAGCC | 32866187 | 32828407 | 21804076 | 17105387 |
MFM1 | Adult fed male midgut/fat body | 1 | TCCGGAGA | CTTCGCCT | 33881743 | 33839530 | 23694857 | 18773687 |
MFM2 | Adult fed male midgut/fat body | 2 | TCCGGAGA | TAAGATTA | 30640838 | 30616043 | 23809219 | 19162578 |
MFM3 | Adult fed male midgut/fat body | 3 | TCCGGAGA | ACGTCCTG | 29838624 | 29814207 | 21278225 | 17167448 |
MFM4 | Adult fed male midgut/fat body | 4 | TCCGGAGA | GTCAGTAC | 22832064 | 22820560 | 17562596 | 14236426 |
MUF1 | Adult unfed female midgut/fat body | 1 | ATTACTCG | AGGCTATA | 20441625 | 20422181 | 16173705 | 12413351 |
MUF2 | Adult unfed female midgut/fat body | 2 | ATTACTCG | GCCTCTAT | 21174699 | 21158515 | 17072234 | 12896359 |
MUF3 | Adult unfed female midgut/fat body | 3 | ATTACTCG | AGGATAGG | 20730531 | 20708211 | 16352452 | 12801594 |
MUF4 | Adult unfed female midgut/fat body | 4 | ATTACTCG | TCAGAGCC | 18659429 | 18650081 | 14552744 | 11262799 |
MUM1 | Adult unfed male midgut/fat body | 1 | ATTACTCG | CTTCGCCT | 24886800 | 24858754 | 19023568 | 14522591 |
MUM2 | Adult unfed male midgut/fat body | 2 | ATTACTCG | TAAGATTA | 28619496 | 28592550 | 22067707 | 16663228 |
MUM3 | Adult unfed male midgut/fat body | 3 | ATTACTCG | ACGTCCTG | 21795275 | 21769632 | 17025197 | 13146374 |
MUM4 | Adult unfed male midgut/fat body | 4 | ATTACTCG | GTCAGTAC | 26573408 | 26542803 | 20615412 | 15717687 |
| Â | All libraries | Â | Â | Â | 424776657 | 424355325 | 317944928 | 249860592 |