Fig. 1From: Oral immune priming with Bacillus thuringiensis induces a shift in the gene expression of Tribolium castaneum larvaeExperimental design. To induce priming, larvae (15 days after oviposition) were exposed to spore-culture supernatants and a medium control (unconditioned medium) for 24 h. Subsequently, larvae were transferred to naïve diet (flour + PBS) for 4 days and exposed to a lethal concentration of spores or naïve diet (flour + PBS). After 6 h, pools of 32 larvae were sampled in triplicates from each group (6 groups in total) and their RNA was isolated for library preparationBack to article page