Fig. 6
From: Genome-wide survey of miRNAs and their evolutionary history in the ascidian, Halocynthia roretzi
Criterions that were used in this study and its evaluation by application to metazoan miRNA precursors. a Precursor length distribution and their percent of metazoan miRNA precursors that are registered in miRBase. b Loop number per precursor. Loop number was predicted by RNAfold. c Relationship of MFE and precursor length. MFE was calculated by RNAfold. Red line indicates the equation simulated via linear regression analysis. Black line represents the equation with the shifted constant used in this study, and it allowed ~95.3% precursors to pass the criterion, MFE ≤ -0.31 * L + 6.00. d GC content distribution. e Paired bases per precursor. Paired bases per precursor were obtained by RNAfold results