Fig. 8
ATCC 33988 does not encode lasI/lasR. (a) Genome organization of the lasR-lasI fragment of PAO1. To evaluate the presence or absense of the lasI/lasR genes in ATCC 33988, three sets of PCR primers were designed. The red and blue primer pairs amplify regions within the lasR and lasI genes, respectively. Expected PCR product sizes were 295 bp (lasR) and 204 bp (lasI). The third primer pair (black) anneals to the region flanking the lasR/lasI region, which is sequence-conserved in both strains. When both lasR and lasI were present in this region, the expected PCR product size was 2.4 kbp. All PCR products were run on a 1.2% agarose gel. (b) Heat map representing the fold-change in gene expression between PAO1 in alkanes and ATCC 33988 in alkanes. All expression values are log2-transformed. If a gene is not detected in any sample, the corresponding box is shaded grey. If a gene is not present in ATCC 33988, the log2(fold-change) value is assigned to 1. R = total RNA, F = ribosome footprints, P = proteomics