Fig. 1

Annotation and sequencing depth of the 27 newly sequenced mitochondrial genomes. Annotation features are shown on the top of this figure as a linear representation generated by the MITOS WebServer [34]. In the upper panel, protein-coding genes are colored in red, ribosomal genes in green, and tRNA in blue. Each tRNA is identified with the one-letter code for the corresponding amino acid. The sequencing depth of each position of the mitochondrial genomes is indicated below the general annotation. In the lower panel, color gradient corresponds to the number of reads supporting a given nucleotide, according to the legend at the right of the figure. Illumina sequencing reads were aligned to the correspondent mitochondrial genome, using Bowtie 1.0 and counted on the Integrative Genome Viewer (IGV) [35]. Representation of read counts as a color-gradient was generated using R. For comparative purposes, the recently released mitogenomes of H. incognitum, Ancistrus sp. 1, Ancistrus sp.2 and C. nattereri were included