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Fig. 6 | BMC Genomics

Fig. 6

From: Flow cytometry sorting of nuclei enables the first global characterization of Paramecium germline DNA and transposable elements

Fig. 6

Coverage of the MIC assembly by different sequencing samples. a. Global comparison of the sequences in simulated (left) and real (right) PGM and MIC sequencing samples. Depth is calculated by mapping reads to the MIC assembly, and counting the reads in 1-kb non-overlapping windows. The graph shows the density of windows as a heat map color, for each combination of MIC and PGM normalized depth values. b. Representation of the genomic compartments identified by analysis of differential read coverage of the MIC assembly (cf. Methods, DESeq2 analysis and Table 2). The horizontal bars show the percentage of the MIC assembly covered by each sequencing sample, defining three genomic sub-compartments. “MAC-destined” is the genomic compartment covered by MIC, MAC and PGM reads, i.e. windows with no differential coverage according to the DESeq2 analysis; “MIC PGM” is the compartment covered by MIC and PGM reads; “MIC-only” is the compartment covered only by MIC reads. c. Barplots of the normalized DESeq2 read counts, across all windows and all samples (Additional file 2: Table S1) for the “MIC PGM” compartment (left) and the “MIC-only” compartment (right)

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