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Table 1 Concentration of rotavirus required for genome sequence generation

From: A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection

Sample Initial input Post-viral capture Double-stranded cDNA Random amplification Genome sequencing
Ct value Target copies Ct value Target copies Ct value Target copies Ct value Target copies Total reads Number of RoV reads Proportion of RoV reads (%) RoV genome coverage (%)
Neat 15 1 × 108 23 6 × 105 28.53 1 × 104 14.97 1 × 108 6 × 105 3 × 105 54.5 90.4
Dilution (2−5) 20 4 × 106 28 2 × 104 34.9 2 × 102 19.59 6 × 106 7 × 105 2 × 105 20.7 81.3
Dilution (2−10) 25 1 × 105 33 6 × 102 37.44 3 × 101 23.9 3 × 105 8 × 105 5 × 103 0.7 49.3
Dilution (2−15) 30 7 × 103 36 8 × 101 Negative 0 Negative 0 8 × 105 6 × 102 0.07 26.7
Dilution (2−16) 32 1 × 103 Negative 0 Negative 0 Negative 0 0 0 0 0