Sample | Initial input | Post-viral capture | Double-stranded cDNA | Random amplification | Genome sequencing | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Ct value | Target copies | Ct value | Target copies | Ct value | Target copies | Ct value | Target copies | Total reads | Number of RoV reads | Proportion of RoV reads (%) | RoV genome coverage (%) | |
Neat | 15 | 1 × 108 | 23 | 6 × 105 | 28.53 | 1 × 104 | 14.97 | 1 × 108 | 6 × 105 | 3 × 105 | 54.5 | 90.4 |
Dilution (2−5) | 20 | 4 × 106 | 28 | 2 × 104 | 34.9 | 2 × 102 | 19.59 | 6 × 106 | 7 × 105 | 2 × 105 | 20.7 | 81.3 |
Dilution (2−10) | 25 | 1 × 105 | 33 | 6 × 102 | 37.44 | 3 × 101 | 23.9 | 3 × 105 | 8 × 105 | 5 × 103 | 0.7 | 49.3 |
Dilution (2−15) | 30 | 7 × 103 | 36 | 8 × 101 | Negative | 0 | Negative | 0 | 8 × 105 | 6 × 102 | 0.07 | 26.7 |
Dilution (2−16) | 32 | 1 × 103 | Negative | 0 | Negative | 0 | Negative | 0 | 0 | 0 | 0 | 0 |