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Table 1 Concentration of rotavirus required for genome sequence generation

From: A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection

Sample

Initial input

Post-viral capture

Double-stranded cDNA

Random amplification

Genome sequencing

Ct value

Target copies

Ct value

Target copies

Ct value

Target copies

Ct value

Target copies

Total reads

Number of RoV reads

Proportion of RoV reads (%)

RoV genome coverage (%)

Neat

15

1 × 108

23

6 × 105

28.53

1 × 104

14.97

1 × 108

6 × 105

3 × 105

54.5

90.4

Dilution (2−5)

20

4 × 106

28

2 × 104

34.9

2 × 102

19.59

6 × 106

7 × 105

2 × 105

20.7

81.3

Dilution (2−10)

25

1 × 105

33

6 × 102

37.44

3 × 101

23.9

3 × 105

8 × 105

5 × 103

0.7

49.3

Dilution (2−15)

30

7 × 103

36

8 × 101

Negative

0

Negative

0

8 × 105

6 × 102

0.07

26.7

Dilution (2−16)

32

1 × 103

Negative

0

Negative

0

Negative

0

0

0

0

0