Table 5 Sequence coverage of rotavirus genomes using viral capture and amplification with random and specific primers
Sample ID | GP type | Genome Coverage (%) | VP7 | VP4 | VP6 | VP1 | VP2 | VP3 | NSP1 | NSP2 | NSP3 | NSP4 | NSP5 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Segment length and coverage (%) | |||||||||||||
VN-0172 | G1P[8] | 94.93 | 1008 (94.9) | 2141 (90.6) | 1356 (100) | 3241 (98.2) | 2690 (100) | 2230 (86.1) | 1566 (97.2) | 1059 (100) | 1074 (97.3) | 570 (75.9) | 640 (96.0) |
VN-0341 | G1P[8] | 93.05 | 1012 (95.3) | 2338 (99.0) | 1356 (100) | 3203 (97.0) | 2644 (98.3) | 2513 (97.0) | 1260 (78.2) | 1058 (99.9) | 985 (89.2) | 581 (77.4) | 315 (47.2) |
VN-0221 | UT | 92.52 | 995 (93.7) | 2016 (85.4) | 1168 (86.1) | 3261 (98.8) | 2564 (95.3) | 2266 (87.5) | 1482 (92.0) | 1059 (100) | 956 (86.6) | 748 (99.6) | 652 (97.8) |
VN-0181 | UT | 91.07 | 800 (75.3) | 1678 (71.0) | 1356 (100) | 3222 (97.6) | 2593 (96.4) | 2328 (89.9) | 1418 (88.0) | 1059 (100) | 908 (82.2) | 746 (99.3) | 667 (100) |
VN-0058 | G26P[19] | 92.26 | 1062 (100) | 2337 (98.9) | 1309 (96.5) | 3250 (98.4) | 2321 (86.3) | 2183 (84.3) | 1247 (77.4) | 1001 (94.5) | 1037 (93.9) | 717 (95.5) | 654 (98.1) |
VN-0132 | G3P[4] | 92.64 | 1060 (99.8) | 2220 (93.9) | 1294 (95.4) | 3090 (93.6) | 2690 (100) | 2086 (80.5) | 1452 (90.1) | 996 (94.1) | 1074 (97.3) | 626 (83.4) | 562 (84.3) |
VN-0006 | G1P[8] | 95.87 | 1060 (99.8) | 2338 (99.0) | 1356 (100) | 3050 (92.4) | 2690 (100) | 2591 (100) | 1424 (88.4) | 1029 (97.2) | 1029 (93.2) | 652 (86.8) | 486 (72.8) |