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Table 5 Sequence coverage of rotavirus genomes using viral capture and amplification with random and specific primers

From: A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection

Sample ID GP type Genome Coverage (%) VP7 VP4 VP6 VP1 VP2 VP3 NSP1 NSP2 NSP3 NSP4 NSP5
Segment length and coverage (%)
VN-0172 G1P[8] 94.93 1008 (94.9) 2141 (90.6) 1356 (100) 3241 (98.2) 2690 (100) 2230 (86.1) 1566 (97.2) 1059 (100) 1074 (97.3) 570 (75.9) 640 (96.0)
VN-0341 G1P[8] 93.05 1012 (95.3) 2338 (99.0) 1356 (100) 3203 (97.0) 2644 (98.3) 2513 (97.0) 1260 (78.2) 1058 (99.9) 985 (89.2) 581 (77.4) 315 (47.2)
VN-0221 UT 92.52 995 (93.7) 2016 (85.4) 1168 (86.1) 3261 (98.8) 2564 (95.3) 2266 (87.5) 1482 (92.0) 1059 (100) 956 (86.6) 748 (99.6) 652 (97.8)
VN-0181 UT 91.07 800 (75.3) 1678 (71.0) 1356 (100) 3222 (97.6) 2593 (96.4) 2328 (89.9) 1418 (88.0) 1059 (100) 908 (82.2) 746 (99.3) 667 (100)
VN-0058 G26P[19] 92.26 1062 (100) 2337 (98.9) 1309 (96.5) 3250 (98.4) 2321 (86.3) 2183 (84.3) 1247 (77.4) 1001 (94.5) 1037 (93.9) 717 (95.5) 654 (98.1)
VN-0132 G3P[4] 92.64 1060 (99.8) 2220 (93.9) 1294 (95.4) 3090 (93.6) 2690 (100) 2086 (80.5) 1452 (90.1) 996 (94.1) 1074 (97.3) 626 (83.4) 562 (84.3)
VN-0006 G1P[8] 95.87 1060 (99.8) 2338 (99.0) 1356 (100) 3050 (92.4) 2690 (100) 2591 (100) 1424 (88.4) 1029 (97.2) 1029 (93.2) 652 (86.8) 486 (72.8)