Fig. 6

Expression patterns of selected 12 miRNAs (line charts) and 15 predicted target genes (bar charts) in ROC22 and YA05-179 at 0, 12, 48, and 96 h after Sporisorium scitamineum challenge. Gene expression levels were assessed by qRT-PCR. The right and left y axis represented the relative expression of miRNA and its predicted target gene, respectively. The relative expression levels of miRNAs and their predicted target genes were normalized to the 5S rRNA and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression levels, respectively. Each bar represented as means of three replicates (n = 3) ± standard error. Different lowercase letters indicate a significant difference, as determined by Duncan’s new multiple range test (P-value <0.05). The names of miRNA and corresponding targeted gene were listed in the top of each panel. Five out of twelve miRNAs, including miR408-3p, novel_mir_32, novel_mir_133, novel_mir_99, and novel_mir_58, were expressed specifically in ROC22 or YA05-179 after S. scitamineum challenge