Fig. 7

A proposed regulatory network of partial miRNAs in sugarcane after Sporisorium scitamineum challenge. The gene expression profiles of five differentially expressed miRNAs, including miR5671, miR5054, miR5783, miR5221, and miR6478, as well as eight of their target genes, including RPM1 (Sugarcane_Unigene_BMK.42342), CDPK (Sugarcane_Unigene_BMK.34960), PEX5 (CA223872), PKA (CA133877), HSP72 (Sugarcane_Unigene_BMK.31740), ABF (Sugarcane_Unigene_BMK.68798), AUX1 (CA105497), and CTR1 (Sugarcane_Unigene_BMK.73145), were validated by qRT-PCR. The sugarcane buds from ROC22 and YA05-179 inoculated with distilled water and S. scitamineum at 0 and 48 h were used for qRT-PCR samples. The expression profiles of the miRNAs were normalized to the 5S rRNA expression level and represented as means of three replicates (n = 3) ± standard error. The expression profiles of the target genes were normalized to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression level and represented as means of three replicates (n = 3) ± standard error. Different lowercase letters indicate a significant difference, as determined by Duncan’s new multiple range test (P-value <0.05). RPM1, effector-triggered immune receptor; CDPK, calmodulin-independent protein kinase; PEX5, peroxisome 5; PKA, protein kinase A; HSP72, heat shock protein 72; ABF, ABA-responsive element-binding protein; AUX1, auxin transporter; CTR1, constitutive triple-response 1