Fig. 5

Screenshot of the phsABCD gene cluster comparison between different S. enterica serotypes. The screenshot from the Sybil comparative analysis tool highlights the phs operon that encodes the enzymes for the anaerobic production of hydrogen sulfide from thiosulfate, which are used in anaerobic respiration. The comparison shows that of the four genes that are present in S. Typhimurium LT2, two (phsA and phsD) are missing from the two S. Paratyphi A strains AKU 12601 and ATCC 9150 and one (phsD) from the two S. Typhi strains CT18 and Ty2. The corresponding genomic regions were manually checked and confirmed to contain interrupted open reading frames in those genomes without gene calls. Gene designations in red were manually added to the screenshot that was directly copied from Sybil browser