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Table 2 RNA-seq based predicted gene models

From: Transcriptome analysis of smut fungi reveals widespread intergenic transcription and conserved antisense transcript expression

Description

Cuffcompare

Code

U. maydis a

U. maydis a

with FPKM >1

U. hordei a

U. hordei a

with FPKM >1

S. reilianum a

S. reilianum a

with FPKM >1

MIPS genes and genetic elements

 

6902

 

7218

 

6779

 

PASA 5' UTR predictions (contain intron)

 

6016 (371)

 

5701 (262)

 

5971 (239)

 

PASA 3' UTR predictions (contain intron)

 

6064 (146)

 

5749 (133)

 

6051 (76)

 

PASA predicted transcript isoforms b

 

763

 

681

 

466

 

Protein-coding genes and genetic elements

 

7665

7080 (6394)

7899

6555 (5943)

7245

6585 (6184)

Single exon transfrag overlapping a reference exon and at least 10 bp of a reference intron, indicating a possible pre-mRNA fragment

e

1593 (1423)

330 (298)

1327 (1192)

193 (181)

1003 (910)

183 (172)

A transfrag falling entirely within a reference intron

i

101 (88)

39 (37)

131 (96)

9 (9)

128 (90)

29 (25)

Potentially novel isoform (fragment): at least one splice junction is shared with a reference transcript

j

659 (327)

386 (242)

407 (280)

249 (191)

337 (246)

196 (157)

Generic exonic overlap with a reference transcript

o

227 (202)

115 (107)

164 (152)

81 (78)

133 (130)

64 (62)

Possible polymerase run-on fragment (within 2Kbases of a reference transcript)

p

1875 (1287)

755 (650)

1413 (1055)

473 (423)

1435 (1026)

554 (490)

Exonic overlap with reference on the opposite strand

x

8173 (3469)

4500 (2624)

5847 (2640)

2332 (1606)

6842 (3083)

2768 (1949)

Unknown, intergenic transcript

u

3853 (3853)

1832 (1832)

2313 (2313)

898 (898)

2888 (2888)

1331 (1331)

Total Transcripts

 

24146

15,037 (12184)

19501

10790 (9329)

20011

11710 (10370)

  1. aMultiple transcripts annotated by PASA and Cufflinks Cuffcompare can hit the same gene or genetic element; therefore, non-redundant gene hits are indicated in parentheses
  2. bUsing default settings, PASA only predicted novel transcript isoforms that did not alter the coding length by >30%