Skip to main content

Table 3 (A) Read assignment benchmark assessment of MetaShot and Vipie on simulated dataseta consisting of 19 582 500 human (94.5%), 986 114 bacterial (4.8%) and 146 886 viral (0.7%) reads. Vipie percentages are based on random subsampling of 1 000 000 reads and bacterial statistics are not reported as Vipie reports information on bacterial ribosome only (the bacterial genomic DNA is not filtered out, as it might lead to loss of dormant phage sequences). (B) Precision, Recall and F-measure are calculated on the same data. Input reads and assessment script are available on SourceForgeb

From: Vipie: web pipeline for parallel characterization of viral populations from multiple NGS samples

A

Assigned %c

Correctly Assigned %d

Ā 

MetaShot

Vipie

MetaShot

Vipie

Human (host)

99.18

99.27

99.99

99.27

Viruses

ā€ƒFamily

97.74

99.98

98.53

93.39

ā€ƒGenus

97.39

98.99

99.75

93.33

ā€ƒSpecies

97.81

93.66

96.70

92.97

B

Human (host)

Virus

Ā 

MetaShot

Vipie

MetaShot

Vipie

Precision (%)

100.00

100.00

98.30

96.85

Recall (%)

99.97

99.96

98.19

95.36

F-measure (%)

100.00

99.98

98.07

96.08

Unclassified (%)

1.04

0.73

3.94

6.73

  1. a https://recascloud.ba.infn.it/index.php/s/nw4s9hqnF8QkBsK
  2. b https://sourceforge.net/projects/vipie/files/validation/k
  3. cThe percentage refers to the total number of reads assignable to the specific taxonomic rank
  4. dThe percentage refers to the relevant assigned reads