Fig. 1

Validation of subgenome-specific TaSSIVb-D primers using the Chinese Spring nullisomic-tetrasomic lines. a Products from the Chinese Spring (CS), nullisomic-tetrasomic lines (N1AT1B, N1AT1D, N1BT1D and N1DT1B) and wild type (J411) amplified using the three different sets of TaSSIVb-D-specific primer pairs shown in b. TaSSIVb-D is absent from N1DT1B. b Diagram of D subgenome primers. The black rectangle represents the gene sequence; orange arrowheads indicate exon regions; blue rectangles represent the location of each primer