Gene expression profile during proliferation and differentiation of rainbow trout adipocyte precursor cells

BMC Genomics

Table 1 Rainbow trout primer sequences used for qPCR

Gene	Accession number	Primer sequence (5'-3')	Annealing T (°C)
ACSL1	CR363150.p.om.8	F: TGCAATCTAGCAAGGTTCCTTTTG	60
ACSL1	CR363150.p.om.8	R: TCCAAGCAGAAACCCAGTACAGAA	60
GPDH	AF027130.p.om.8	F: ATGACCACAGTCCACGCCTACAC	60
GPDH	AF027130.p.om.8	R: GGCAGGTTAGGTCCACCACTGA	60
IGFBP5	DQ206713	F: ACTTCACGCGCTTCTCCATGGCA	60
IGFBP5	DQ206713	R: CGAGACTCATGATCTATGGGTGGA	60
IGFBP7	DQ146965	F: GCTCCGATGGAGTGACCTATA	60
IGFBP7	DQ146965	R: ACAATGACAGGTGCTGTTGCG	60
PCNA	CA358086.s.om.10	F: ATACGGGCAAACTCTCCTGATGGC	60
PCNA	CA358086.s.om.10	R: CAACGCAGACACACTCGCCCTT	60
cPEPCK	[26]	F: CCCAGTGCCTGTGGGAAAAC	60
cPEPCK	[26]	R: CCACACCGAAAAAGCCGTTC	60
PLIN2	CB494091.p.om.8	F: GATGGCAATGAGGCAGAGAACA	60
PLIN2	CB494091.p.om.8	R: AGGCAGAGTGGCTAAGGGACAG	60
18S	AF308535	F: CGGAGGTTCGAAGACGATCA	60
18S	AF308535	R: TCGCTAGTTGGCATCGTTTAT	60

ISSN: 1471-2164