Table 1 Rainbow trout primer sequences used for qPCR
Gene | Accession number | Primer sequence (5'-3') | Annealing T (°C) |
|---|---|---|---|
ACSL1 | CR363150.p.om.8 | F: TGCAATCTAGCAAGGTTCCTTTTG | 60 |
R: TCCAAGCAGAAACCCAGTACAGAA | |||
GPDH | AF027130.p.om.8 | F: ATGACCACAGTCCACGCCTACAC | 60 |
R: GGCAGGTTAGGTCCACCACTGA | |||
IGFBP5 | DQ206713 | F: ACTTCACGCGCTTCTCCATGGCA | 60 |
R: CGAGACTCATGATCTATGGGTGGA | |||
IGFBP7 | DQ146965 | F: GCTCCGATGGAGTGACCTATA | 60 |
R: ACAATGACAGGTGCTGTTGCG | |||
PCNA | CA358086.s.om.10 | F: ATACGGGCAAACTCTCCTGATGGC | 60 |
R: CAACGCAGACACACTCGCCCTT | |||
cPEPCK | [26] | F: CCCAGTGCCTGTGGGAAAAC | 60 |
R: CCACACCGAAAAAGCCGTTC | |||
PLIN2 | CB494091.p.om.8 | F: GATGGCAATGAGGCAGAGAACA | 60 |
R: AGGCAGAGTGGCTAAGGGACAG | |||
18S | AF308535 | F: CGGAGGTTCGAAGACGATCA | 60 |
R: TCGCTAGTTGGCATCGTTTAT |