Fig. 6

Gene organization of the ETT2 gene cluster in the bovine-associated E. coli genomes. Comparison of the ETT2 gene cluster in the E. coli of the strain panel based on BLASTN+. Homologous regions are connected via grey vertices and colored by nucleotide identity. The genomes are ordered according to the WGA core genome phylogeny (Additional file 3: Figure S2A), which is attached on the left side (bootstrap support values below 50 were removed). Phylogroups are indicated correspondingly. MAEC strain names are colored in light red and commensals in green. Gene names are indicated above genomes encoding for these. The respective contigs of the draft genomes containing the gene cluster were concatenated (contig boundaries are indicated by red vertical lines) and CDS spanning contig borders reannotated if needed (indicated by asterisks). ETT2 contigs of genome D6-117.29 were difficult to concatenate, because of its high fragmentation. Backbone genes not belonging to ETT2 are colored black. Genes within the ETT2 region have different colors (see the legend) to be able to evaluate their presence. Pseudogenes have a lighter color fill. ETT2 shows a large number of different mutational isoforms. Nevertheless, ETT2 composition follows phylogenetic history rather than pathotype affiliation