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Table 1 DE miRNAs revealed by DESeq2 and RT-qPCR analysis in SAV3-i4 infected individuals at week 4 poc

From: Identification of differentially expressed Atlantic salmon miRNAs responding to salmonid alphavirus (SAV) infection

Mature miRNA

SAV expression1

log2 fold change2

DESeq2 P-adj.3

log2 f.c. RT-qPCR4

RT-qPCR P-adj.5

Family6

ssa-miR-462a-5p

increase 13x

3.71

1.93x10-5

2.58

3.0x10-3

462

ssa-miR-462a-3p

increase 19x

4.30

2.41x10-5

3.12

3.0x10-3

462

ssa-miR-462b-5p

increase 3x

1.75

0.07

462

ssa-miR-731-5p

increase 12x

3.58

1.79x10-3

2.63

3.0x10-3

731

ssa-miR-731-3p

increase 8x

3.04

1.64x10-3

2.28

3.0x10-3

731

ssa-miR-146b-5p

increase 14x

3.82

1.17x10-4

2.38

3.0x10-3

146

ssa-miR-146a-3p

increase 6x

2.64

0.03

2.99

3.0x10-3

146

ssa-miR-146a-3-3p

increase 8x

3.00

0.03

146

ssa-miR-21a-1-3p

increase 10x

3.27

4.3x10-3

3.31

3.0x10-3

21

ssa-miR-21a-2-3p

increase 8x

3.16

0.02

2.21

3.0x10-3

21

ssa-miR-21b-3p

increase 5x

2.36

0.06

2.33

3.0x10-3

21

ssa-miR-181c-5p

increase 4x

2.14

0.09

1.42

0.011

181

ssa-miR-223-5p

increase 5x

2.36

0.06

2.46

3.0x10-3

223

ssa-miR-1338-3p

increase 3x

1.65

0.06

1.61

3.0x10-3

1338 (1388)

ssa-miR-155-5p

increase 3x

1.72

0.06

0.58

0.1

155

ssa-miR-92a-3-5p3

increase 10x

3.4

0.06

92

ssa-miR-7132-3p

increase 3x

1.79

0.06

1.88

3.0x10-3

7132

ssa-miR-7132-5p

increase 4x

2.11

0.07

1.06

0.02

7132

ssa-miR-734-3p

decrease 4x

-2.09

0.07

-0.19

0.96

734

ssa-miR-2188-3p

decrease 2x

-1.08

0.10

-0.95

3.0x10-3

2188

  1. 1Direction and approximate relative change (times fold change) of mature miRNAs in SAV infected tisssues from DESeq2 measurements
  2. 2Log2 fold change from DESeq2 analysis of deep sequencing samples
  3. 3Adjusted p-values from DESeq2 analysis. 0.1 was used as threshold for significance
  4. 4Log2 fold change (∆∆Ct-method) from RT-qPCR analysis
  5. 5 P-values from RT-qPCR after adjustment according to Benjamini-Hochberg procedure. 0.05 was used as threshold for significance
  6. 6Those with identical numbers belong to same miRNA gene family or are corresponding 5p and 3p from same miRNA gene