Table 2 Results from prediction of target genes among a set of SAV responsive immune network genes
Gene | Gene function/relevance to SAV infection1 | Predicted by four search tools2 | Predicted by three search tools3 | Reference4 |
|---|---|---|---|---|
Neutrophil cytosolic factor 1 | inflammatory response, superoxide anion and cell death, increased expression in SAV | miR-92a-3-5p, miR-181c-5p | miR-146a-3-3p, miR-146a-3p, miR-155-5p | Johansen et al., [38] |
VHSV inducible like protein (VIG-B319) | virus responsive, increased expression in VHSV and SAV | miR-155-5p, miR-223-5p | miR-146b-5p, miR-1338-3p | Johansen et al., [38] |
Jeltraxin | similar to CRP, innate immunity pathway, increased expression in SAV | miR-146b-5p | miR-181c-5p | Johansen et al., [38] |
Interferon-inducible protein Gig2-7-like | virus responsive, increased expression in SAV | − | miR-462a-3p | Johansen et al., [38] |
Nuclear factor of kappa light polypept gene enhancer in B-cells 2 (NFkB2) | miR-223 target in other vertebrate, innate immune system, inflammatory response | miR-462a-3p | miR-146b-5p | Aziz et al., [43] |
Myeloperoxidase | promote inflammation, innate immunity pathway, increased expression in SAV | miR-462a-3p, miR-146b-5p | miR-223-5p, miR-734-3p, miR-2188-3p | Johansen et al., [38] |
Macrosialin precursor CD68 | virus responsive, increased expression in SAV | miR-462a-3p, miR-731-5p | miR-181c-5p | Johansen et al., [38] |
ATP-dependent RNA helicase DHX58 (RIG-I-like 3) | virus responsive, innate immunity pathway, increased expression in SAV | − | miR-731-5p | Johansen et al., [38] |
Interferon regulatory factor 7 (IRF7) | transcription factor activating innate immunity pathway, increased expression in SAV | miR-731-5p | − | Xu et al., [39] |
Interferon regulatory factor 3 (IRF3) | transcription factor activating innate immunity pathway, increased expression in SAV | miR-21a-2-3p, miR-21b-3p | miR-181c-5p, miR-734-3p | Xu et al., [39] |
C–C motif chemokine 19 | inflammatory response, innate immunity pathway, increased expression in SAV | − | miR-731-3p, miR-223-5p, miR-1338-3p | Johansen et al., [38] |
Interferon-inducible protein Gig2-2-like | virus responsive, increased expression in SAV | miR-181c-5p | − | Johansen et al., [38] |
Inhibitor of nuclear factor kappa-B kinase subunit alpha-like (IKKa) | miR-223 target in other vertebrate, innate immune system and inflammatory response | miR-181c-5p (multiple sites) | miR-223-5p, miR-7132-5p, miR-1338-3p, miR-734-3p | Aziz et al., [43] |
Cholesterol 25-hydroxylase-like protein A | virus responsive, innate immunity pathway, increased expression in SAV | miR-181c-5p | − | Johansen et al., [38] |
Complement factor D (CFAD) | inflammatory response, innate immunity pathway, upregulated in SAV | − | miR-223-5p | Johansen et al., [38] |
Interleukin-10 receptor beta chain precursor | Cytokine-mediated signaling pathway, immune response, upregulated in SAV | − | miR-21a-2-3p, miR-146a-3-3p, miR-146a-3p, miR-223-5p | Johansen et al., [38] |
SOCS1 | antiviral innate immune response | − | miR-223-5p | Taganov et al., [45] |
Fish virus induced TRIM-3 | Virus responsive, antiviral innate immunity, upregulated in SAV | − | miR-181c-5p | Johansen et al., [38] |
Forkhead box protein O1-A-like (FOXO1) | miR-223 target in other vertebrate, immune system signaling pathways, promote cell death | − | miR-2188-3p | Aziz et al., [43] |
Complement C1qB subcomponent | inflammatory response, innate immunity pathway, increased expression in SAV | − | miR-21a-1-3p, miR-223-5p, miR-2188-3p (multiple sites) | Johansen et al., [38] |
Barrier-to-autointegration factor | known to be exploited by retrovirus, increased expression in SAV | − | miR-21a-2-3p, miR-21b-3p | Johansen et al., [38] |
Viperine (RSAD2) | virus responsive, innate immunity antiviral, increased expression in SAV | − | miR-223-5p, miR-734-3p | Johansen et al., [38] |
Interferon gamma 1 | virus responsive, cytokine | − | miR-734-3p | Sun et al., [40] |
Interferon gamma 2 | virus responsive, cytokine | − | miR-734-3p | Sun et al., [40] |