Fig. 4
From: GUIDEseq: a bioconductor package to analyze GUIDE-Seq datasets for CRISPR-Cas nucleases
Venn Diagram generated using combineOfftargets to depict the overlaps of off-target sites between three different nuclease variants. Example of the output from the combineOfftargets function (Example 6) comparing the overlap in GUIDE-seq identified off-target sites for wild-type Cas9, Split-Cas9 (dual NLS) [51], and the highly specific SpCas9MT3-ZFP [25] programmed with a sgRNA recognizing VEGFA site 2 (TS2) [11]