Skip to main content
Fig. 1 | BMC Genomics

Fig. 1

From: Drosophila melanogaster positive transcriptional elongation factors regulate metabolic and sex-biased expression in adults

Fig. 1

Experimental design and efficiency of RNAi or Cdk9 DN expression. a Experimental design. The cartoons (top) illustrate the sham genotypes where Gal80ts (red) and Gal4 (green) are produced in the absence of a responding UAS transgene (left), or when the UAS transgenes are present at the uninducing (middle) or inducing (right) temperatures. We used homozygous P{tubP-GAL4} LL7, P{tubP-GAL80 ts}7 virgin females for all crosses. Sham flies were produced by crossing to lacZ (Sham1, yellow fill) or w 1118 (Sham2, gray fill) males. The schemata (bottom) shows how the shams were used as references for all induction timepoints for both females and males in results presented in subsequent figures. See Methods for further information. Transgenes are shown as bars, with regulatory sequences (open) from tubulin (tub) or the Gal4 upstream activation sequence (UAS), and encoding sequences (filled) from the Gal80 ts (red), Gal4 (green), shRNAi, and Cdk9 DN (black) labeled. Gal80ts (red hexagons), Gal4 (green ovals) proteins are shown. Active (bent black arrows) and repressed (red X) transcription are indicated. b-d Histograms showing expression of lilli (b), Su(Tpl) (c), and Cdk9 (d) transcripts based on normalized read counts (linear scale from DESeq2 [62]) across the gene models after zero (0d), one (1d), or two (2d) days of induction. Biological replicate numbers (#) are indicated. Values for females (left) and males (right) are shown in each panel. Knockdown expression levels following induction of lilli RNAi, Su(Tpl) RNAi, and Cdk9 DN (black), and shams (yellow or gray) as well as one standard deviation (bars) are shown. Significant differential expression (p adj < 0.05 from DESeq2) relative to sex and timepoint matched shams are shown (asterisk)

Back to article page