Fig. 2

Differential expression and clustering. a-b Vocano-plots showing examples of differential expression for lilli ^RNAi (a) and Su(Tpl) ^RNAi (b) one day after induction in female samples relative to Sham2 versus significance (truncated at > 75). Genes with significant differential expression (DESeq2 p adj < 0.05, red) and no significant differential expression (DESeq2 p adj ≥ 0.05, black) are shown. Gene expression are shown as log₂ratios of medians (X tilde) of experimental (exp) replicates divided by sham replicates. c Differential expression heatmap of experimental samples relative to shams. Values indicated in the key (bottom) were truncated at +/- 2. Reduced (blue) and increased (red) expression in experimental samples relative to sham are shown. 579 genes (rows) with coherent differential expression relative to shams are shown. Sample comparisons (columns) relative to Sham1 (yellow fill) or Sham2 (gray fill) are indicated (bottom), as are female (f) and male (m) sex, timepoint in day(s) (d), and tested transgenes. The k-means cluster ID used in the text is shown (right) and highlighted within the heatmap (open black boxes)