Fig. 2

Genome-wide expression profiling of TM, TM-MSC, cornea and sclera. a Venn diagram indicates number of genes elevated in the TM and TM-MSC with respect to each other as determined by their comparative analysis (left). Venn diagram of genes differentially expressed between TM vs cornea and TM vs sclera (right). 22 genes were differentially expressed in the TM relative to cornea and sclera. (Statistical threshold of fold change ≥ 2.0 and P _adj ≤ 0.05 were applied for both). b Hierarchical clustering of the sample sets based on whole transcriptome profile showed that the tissues shared some extent of expression similarity, with sclera sharing closer similarity to the TM. The TM-MSC profile was divergent from the tissues. c Venn diagram designed by merging the gene lists (TM vs TM-MSC/cornea/sclera) found 18 genes to be differentially expressed in the TM compared to the other sample sets under the statistical threshold selected