Fig. 3

Evolutionary history of pPT23A family plasmid and their Pseudomonas syringae and related Pseudomonas hosts. a Phylogenetic analysis of the complete repA sequences of pPT23A family plasmids from Pseudomonas syringae and related strains, using as the outgroup the repA sequences of two plasmids (p33 and p64) from Xanthomonas citri pv. citri A306. b Phylogenetic analysis using the repA sequences from Pseudomonas syringae plasmids, not including in this tree the outgroup plasmids. c Phylogeny of Pseudomonas syringae and related strains harboring plasmids from the pPT23A family was constructed based on the analysis of two concatenated housekeeping genes (gyrB and rpoD), using as the outgroup the gyrB-rpoD sequences from Xanthomonas citri pv. citri A306. d Phylogenetic analysis using the concatenated housekeeping genes (gyrB and rpoD) from Pseudomonas syringae and related strains, not including in this tree the outgroup strain. The neighbor-joining tree was constructed by MEGA 5 using the Jukes-Cantor model. Percent bootstrap values of more than 50% (1000 repetitions) are shown at the nodes. The topology of the different trees, was identical by using the minimum evolution and maximum parsimony methods. Gray boxes: the outgroup plasmids and strain. Black boxes: plasmids sequenced in this study. Yellow Boxes: corresponding with P. syringae strains that harbor two pPT23A plasmids, with each plasmid belonging to a different evolutionary cluster