Complete sequence and comparative genomic analysis of eight native Pseudomonas syringae plasmids belonging to the pPT23A family

Table 4 Hypothetical proteins predicted as bacterial secreted effector proteins. Detection of signal peptides, transmembrane domains and sequence based prediction

H. proteins^a	Protein size (aa)	Signal peptide (from-to)	Trans. domains (from-to)	Type III effector prediction with standard set^b	Type III effector prediction with plant set^c
pPs0170
Hp11	79	1 (34–46)	ND^d	0.99817	NP^e
pPs7B44
Hp6	191	1 (26–38)	ND	0.94647	NP
pPg2708
Hp10	242	1 (55–67)	ND	0.99997	1
Hp18	160	1 (14–26)	ND	0.99922	1
Hp27	94	1 (7–19)	ND	0.99988	0.99994
pPt0893-29
Hp4	66	1 (1–9)	ND	0.99903	0.9804
Hp14	62	1 (1–8)	ND	0.99447	NP
Hp27	140	1 (60–72)	ND	NP	0.99798

^a Hypothetical proteins that meet the three requirements, in all the plasmids analyzed
^b Standard classification module: comprises effectors of Escherichia coli, Salmonella, Chlamydia, Yersinia, and Pseudomonas
^c Plant classification module: comprises effector sequences from Pseudomonas syringae
^d ND Non-detected
^e NP Non-predicted

ISSN: 1471-2164