Skip to main content
Fig. 5 | BMC Genomics

Fig. 5

From: Transcription factor-associated combinatorial epigenetic pattern reveals higher transcriptional activity of TCF7L2-regulated intragenic enhancers

Fig. 5

Functional validations of the predicted TCF7L2 enhancers by T-cep. a Quantitative PCR analysis of selected gene associated with TCF7L2-enhancers in MCF7 and PANC1 cells after TCF7L2 knockdown. Selected genes contain both of TCF7L2 intragenic and distal enhancers for further analysis. All data are normalized by GAPDH mRNA and represent the average of three independent experiments with p-values less than 0.05 (t-test). b Luciferase reporter activity assay in TCF7L2 knockdown cells. MCF7 and PANC1 cells are transient transfected with intragenic or distal TFE-pGL3 vector at the TCF7L2-siRNA or non-targeting siRNA conditions. c Luciferase reporter activity assay in TCF7L2 overexpression cells. Intragenic or distal TF-enhancer-pGL3 vector and TCF7L2-pcDNA or empty vector are transient transfected into MCF7 and PANC1 cells

Back to article page