Fig. 4

Northern blot analysis of (a) Aspergillus fumigatus chrysovirus (AfuCV); (b) Aspergillus fumigatus tetramycovirus-1 (AfuTmV-1) and (c) Aspergillus fumigatus partitivirus-1 (AfuPV-1) derived sRNAs. Virus-free isolates (−) and virus-infected isolates (+) are shown separately. Probes were labelled with P³² and the membrane was hybridised at 37 °C overnight prior to exposure to a phosphorimaging plate (GE Life Sciences) at 4 °C overnight using a Typhoon FLA 7000 Phosphorimager (GE Life Sciences). The same membrane was used for each probe following removal of the original probe by heating for 2 h at 90 °C