Fig. 2

Principal component analysis (PCA) (a) and hierarchical cluster analysis (b) of gene expression variation among 27 RNA-Seq libraries obtained from midguts of sugarfed, SM fed, and PM fed mosquitoes. For PCA, data visualization and plotting were performed using R. The Euclidean distance measure was used to calculate distances and data fitting was performed by Ward's method [55]. SM, saline meal (PBS); PM, protein meal (BSA)