Table 2 Major altered metabolic pathways in the ΔVpacspA, ΔVpacspD and ΔVpacspAD mutants of V. parahaemolyticus CHN25 grown at the low temperature

Valine, leucine and isoleucine degradation

VpaChn25A_0480

2.4983

Acetyl-CoA acetyltransferase

VpaChn25A_0554

3.0843

Hydroxymethylglutaryl-CoA lyase

VpaChn25A_0555

3.2361

Acyl-CoA carboxylase alpha chain

VpaChn25A_0556

2.4095

Enoyl-CoA hydratase/isomerase

VpaChn25A_0557

2.662

Acyl-CoA carboxyltransferase beta chain

VpaChn25A_0558

2.216

Acyl-CoA dehydrogenase

VpaChn25A_0560

2.8734

Acyl-CoA thiolase

VpaChn25A_0561

7.0543

Aldehyde dehydrogenase

VpaChn25A_0565

7.3736

3-hydroxyisobutyrate dehydrogenase

VpaChn25A_1033

2.7479

Methylmalonate-semialdehyde dehydrogenase

VpaChn25A_1036

2.1029

Acyl-CoA dehydrogenase

Propanoate metabolism

VpaChn25_1376

6.0834

4-aminobutyrate aminotransferase

VpaChn25_1635

6.3526

PrpE protein

VpaChn25_1638

8.5787

Methylcitrate synthase

VpaChn25_1639

6.7065

2-methylisocitrate lyase

VpaChn25_2798

3.959

Acetyl-CoA synthetase

ABC transporters

VpaChn25A_0128

0.4376

ABC transporter substrate-binding protein

VpaChn25A_0130

0.428

ABC transporter ATP-binding protein

VpaChn25A_0533

3.1521

High-affinity branched-chain amino acid transport ATP-binding protein

VpaChn25A_0535

3.9903

ABC transporter membrane spanning protein

VpaChn25A_0536

5.0642

High-affinity branched-chain amino acid transport permease

VpaChn25A_0537

6.8795

Hypothetical protein

VpaChn25A_0538

4.7093

High-affinity branched-chain amino acid transport ATP-binding protein

VpaChn25A_0571

0.3015

Glycine betaine-binding ABC transporter

VpaChn25A_0572

0.3358

Permease

VpaChn25A_0573

0.3801

ABC transporter ATP-binding protein

VpaChn25A_0595

0.4067

Ribose ABC transporter permease

VpaChn25A_0604

0.406

Iron (III) ABC transporter periplasmic iron-compound-binding protein

VpaChn25A_1325

0.2076

Iron (III) ABC transporter ATP-binding protein

VpaChn25A_1326

0.2262

Iron (III) ABC transporter periplasmic iron-compound-binding protein

VpaChn25A_1327

0.3019

Iron-hydroxamate transporter permease subunit

VpaChn25A_1333

2.1634

Transport protein

VpaChn25A_1544

0.2026

Iron-dicitrate transporter substrate-binding subunit

VpaChn25_0306

0.4761

Thiamine transporter membrane protein

VpaChn25_0363

2.1743

ABC transporter substrate binding protein

VpaChn25_0846

0.4129

Zinc ABC transporter permease

VpaChn25_0848

0.4556

Zinc ABC transporter periplasmic zinc-binding protein

VpaChn25_1344

0.2076

Oligopeptide ABC transporter ATP-binding protein

VpaChn25_1345

0.2716

Oligopeptide ABC transporter ATP-binding protein

VpaChn25_1346

0.2355

Oligopeptide ABC transporter permease

VpaChn25_1347

0.3245

Oligopeptide ABC transporter permease

VpaChn25_1348

0.3723

Oligopeptide ABC transporter periplasmic oligopeptide-binding protein

VpaChn25_1613

2.4584

Amino acid ABC transporter substrate-binding protein

VpaChn25_1714

0.4917

Glycine/betaine/proline ABC transporter

VpaChn25_1913

2.222

Hypothetical protein

VpaChn25_2428

0.4529

Iron (III) ABC transporter permease

VpaChn25_2429

0.3234

Iron (III) ABC transporter periplasmic iron-compound-binding protein

VpaChn25_2987

0.2357

Amino acid ABC transporter permease

VpaChn25_2988

0.1424

Amino acid ABC transporter substrate-binding protein

Bacterial secretion systems

VpaChn25A_0952

4.5459

Hypothetical protein

VpaChn25A_0954

6.1398

ClpA / B-type chaperone

VpaChn25A_0966

6.0405

Hypothetical protein

VpaChn25A_0967

7.0563

Hypothetical protein

VpaChn25A_0969

2.4559

Hypothetical protein

VpaChn25_1662

0.3404

Type III secretion system protein

VpaChn25_1663

0.2481

Type III secretion system protein

VpaChn25_1664

0.2099

Translocation protein in type III secretion

VpaChn25_1665

0.2746

Translocation protein in type III secretion

VpaChn25_1666

0.2109

Translocation protein in type III secretion

VpaChn25_1887

0.4446

Outer membrane protein TolC

Ascorbate and aldarate metabolism

VpaChn25A_0230

0.1973

PTS system ascorbate-specific transporter subunit IIC

VpaChn25A_0231

0.1668

Sugar phosphotransferase component II B

VpaChn25A_0232

0.1096

Phosphotransferase enzyme II, A component

Sulfur metabolism

VpaChn25_0788

0.2523

Cysteine synthase A

VpaChn25_0937

3.0238

Cysteine synthase / cystathionine beta-synthase family protein

VpaChn25_1397

2.7136

Homoserine O-succinyltransferase

VpaChn25_2650

0.4424

Phosphoadenosine phosphosulfate reductase

VpaChn25_2651

0.4934

Sulfite reductase subunit beta

VpaChn25_2652

0.2017

Sulfite reductase (NADPH) flavoprotein subunit alpha

VpaChn25_2692

2.1317

Cystathionine gamma-synthase

Glycerophospholipid metabolism

VpaChn25A_0425

0.4957

Diacylglycerol kinase

VpaChn25A_0732

2.2688

Outer membrane phospholipase A

VpaChn25_0642

0.4375

Phosphatidylglycerophosphatase A

VpaChn25_0885

0.3795

Surfactin synthetase

VpaChn25_2245

0.2175

Glycerophosphodiester phosphodiesterase

VpaChn25_2251

0.2013

Glycerol-3-phosphate dehydrogenase

PTS

VpaChn25A_0230

0.2003

PTS system ascorbate-specific transporter subunit IIC

VpaChn25A_0231

0.1886

Sugar phosphotransferase component II B

VpaChn25A_0232

0.1253

Phosphotransferase enzyme II, A component

VpaChn25A_0303

12.8135

PTS system fructose-specific transporter subunit IIA

VpaChn25A_1196

0.3193

Mannitol-specific PTS system enzyme II component

VpaChn25A_1309

3.4141

PTS system fructose-specific transporter subunit IIB

VpaChn25A_1312

4.9223

PTS system fructose-specific transporter subunit IIABC

VpaChn25_0668

0.4181

PTS system trehalose (maltose)-specific transporter subunits IIBC

VpaChn25_2564

2.1655

PTS system cellobiose-specific transporter subunit IIA

VpaChn25_2566

2.8755

PTS system cellobiose-specific transporter subunit IIB

Alanine, aspartate and glutamate metabolism

VpaChn25A_0370

3.4733

Adenylosuccinate synthase

VpaChn25_0104

2.2225

Glutamine synthetase

VpaChn25_0345

0.3501

Glucosamine-fructose-6-phosphate aminotransferase

VpaChn25_0436

2.1782

Glutamate synthase subunit beta

VpaChn25_0437

2.0222

Glutamate synthase subunit alpha

VpaChn25_1375

3.4554

Succinate-semialdehyde dehydrogenase

VpaChn25_1376

3.0672

4-aminobutyrate aminotransferase

VpaChn25_2552

0.4804

Glutaminase

VpaChn25_2583

2.0986

Aspartate carbamoyltransferase

VpaChn25_2584

2.7419

Aspartate carbamoyltransferase

VpaChn25_2784

0.4744

Aspartate ammonia-lyase

Arginine and proline metabolism

VpaChn25_1371

2.2205

Aldehyde dehydrogenase

VpaChn25_1372

2.779

Oxidoreductase

VpaChn25_1373

3.3592

Carbon-nitrogen hydrolase

VpaChn25_1374

3.206

Hypothetical protein

VpaChn25_2581

3.3917

Arginine deiminase

VpaChn25_2719

2.8035

Succinylglutamic semialdehyde dehydrogenase

VpaChn25_2720

3.9099

Arginine/ornithine succinyltransferase

VpaChn25_2721

2.1486

Bifunctional N-succinyldiaminopimelate-aminotransferase/acetylornithine transaminase protein

Valine, leucine and isoleucine degradation

VpaChn25A_0480

3.4643

Acetyl-CoA acetyltransferase

VpaChn25A_0555

2.023

Acyl-CoA carboxylase alpha chain

VpaChn25A_0557

2.0591

Acyl-CoA carboxyltransferase beta chain

VpaChn25A_0561

4.081

Aldehyde dehydrogenase

VpaChn25A_0565

3.2953

3-hydroxyisobutyrate dehydrogenase

VpaChn25A_1038

0.4859

Enoyl-CoA hydratase / isomerase

VpaChn25_0020

2.6571

Multifunctional fatty acid oxidation complex subunit alpha

VpaChn25_2076

0.4816

3-ketoacyl-CoA thiolase

Propanoate metabolism

VpaChn25_1635

2.316

PrpE protein

VpaChn25_1638

4.6552

Methylcitrate synthase

VpaChn25_1639

3.654

2-methylisocitrate lyase

VpaChn25_2433

3.0153

Bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

VpaChn25_2798

2.1035

Acetyl-CoA synthetase

Nitrogen metabolism

VpaChn25A_0296

4.3124

Oxidoreductase protein

VpaChn25A_0917

3.7442

Nitrite reductase large subunit

VpaChn25A_1392

0.4447

Carbonic anhydrase

VpaChn25_1817

9.4056

Cytochrome c552

VpaChn25_2448

0.2912

Carbonic anhydrase

TCA

VpaChn25_0313

0.2603

Malate dehydrogenase

VpaChn25_0837

0.1445

Type II citrate synthase

VpaChn25_0838

0.2716

Succinate dehydrogenase cytochrome b556 large membrane subunit

VpaChn25_0840

0.402

Succinate dehydrogenase flavoprotein subunit

VpaChn25_0841

0.3554

Succinate dehydrogenase iron-sulfur subunit

VpaChn25_0843

0.3915

Dihydrolipoamide succinyltransferase

VpaChn25_0844

0.3535

Succinyl-CoA synthetase subunit beta

VpaChn25_0845

0.3009

Succinyl-CoA synthetase subunit alpha

VpaChn25_1035

0.4921

Isocitrate dehydrogenase

VpaChn25_2762

0.478

Fumarate reductase flavoprotein subunit

VpaChn25_2763

0.4836

Fumarate reductase iron-sulfur subunit

VpaChn25_2764

0.4898

Fumarate reductase subunit C

VpaChn25_2765

0.3058

Fumarate reductase subunit D

PTS

VpaChn25A_0230

0.3036

PTS system ascorbate-specific transporter subunit IIC

VpaChn25A_0231

0.2665

Sugar phosphotransferase component II B

VpaChn25A_0232

0.232

Phosphotransferase enzyme II, A component

VpaChn25A_0303

8.0738

PTS system fructose-specific transporter subunit IIA

VpaChn25A_1312

8.7743

PTS system fructose-specific transporter subunit IIABC

VpaChn25_0356

0.3818

PTS system mannitol-specific transporter subunit IIABC

VpaChn25_2566

0.3343

PTS system cellobiose-specific transporter subunit IIB

VpaChn25_0668

3.948

PTS system trehalose (maltose)-specific transporter subunits IIBC

Butanoate metabolism

VpaChn25A_0528

2.6111

Acetoacetyl-CoA synthetase

VpaChn25A_0560

2.8181

Acyl-CoA thiolase

Fructose and mannose metabolism

VpaChn25A_1313

5.1663

Mannose-6-phosphate isomerase

VpaChn25_0355

0.3626

Mannitol-1-phosphate 5-dehydrogenase

Pyruvate metabolism

VpaChn25A_0307

2.3933

Hypothetical protein

VpaChn25A_0367

0.321

Phosphoenolpyruvate synthase

VpaChn25A_0934

0.4136

D-lactate dehydrogenase

VpaChn25_0112

0.1659

Phosphoenolpyruvate carboxykinase

VpaChn25_1693

2.2321

Aldehyde dehydrogenase

VpaChn25_1927

0.408

Pyruvate kinase II

Oxidative phosphorylation

VpaChn25A_0546

2.1237

Cytochrome BD2 subunit II

VpaChn25_1519

0.4848

Cytochrome c oxidase subunit CcoP

VpaChn25_1521

0.4651

cbb3-type cytochrome c oxidase subunit II

Cysteine and methionine metabolism

VpaChn25_0576

0.293

Homocysteine synthase

VpaChn25_0788

0.2948

Cysteine synthase A

VpaChn25_0937

2.3269

Cysteine synthase/cystathionine beta-synthase family protein

VpaChn25_1880

2.8826

5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase

VpaChn25_2471

0.4433

S-ribosylhomocysteinase

VpaChn25_2646

2.2961

Aspartate kinase

Arginine and proline metabolism

VpaChn25A_1611

0.4145

Bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase

VpaChn25_1332

0.1954

Hydroxyproline-2-epimerase

VpaChn25_1335

0.3527

Ornithine cyclodeaminase

VpaChn25_1544

0.3291

NAD-glutamate dehydrogenase

VpaChn25_2719

0.3969

Succinylglutamic semialdehyde dehydrogenase

VpaChn25_2720

0.482

Arginine /ornithine succinyltransferase

VpaChn25_2721

0.2687

Bifunctional N-succinyldiaminopimelate-aminotransferase / acetylornithine transaminase protein

Alanine, aspartate and glutamate metabolism

VpaChn25_0345

0.2743

Glucosamine-fructose-6-phosphate aminotransferase

VpaChn25_0438

2.461

Glutamate synthase subunit beta

VpaChn25_0439

2.6963

Glutamate synthase, large subunit

VpaChn25_1114

0.407

Alanine dehydrogenase

VpaChn25_2022

0.4771

Cytoplasmic asparaginase I