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Table 2 Summary of sequencing and transcriptome assembly results

From: Genomic adaptation to agricultural environments: cabbage white butterflies (Pieris rapae) as a case study

Sequencing (for de novo transcriptome)

 Raw reads (101 nt paired-end)

179472918 pairs

 Cleaned reads

159812653 pairs

14974970 orphans

 Sequenced bases (cleaned)

31.8 Gb

Assembly

 Number of transcripts (contigs)

31624

 Number of unigenes

17595

 Mean length (unigene)

1420.69 bp

 Median length (unigene)

909 bp

 N50 (unigene)

2416 bp

 Assembled length (unigenes)

25.0 Mb

 GC content (unigenes)

38.8%

 Number of protein-coding ORFs

13991

 Mean ORF length

1058.4 bp

BUSCO arthropod gene set (2675 genes)

 Complete, single-copy

1875 (70.1%)

 Complete, duplicated

72 (2.7%)

 Partial

165 (6.2%)

 Missing

563 (21.0%)