Fig. 5

Sequence data from S. equi mutant libraries generated with ISS1. a. Overview of the integrative conjugative element, ICESe2. Green and red peaks indicate reads mapping on the forward and reverse strand, respectively. ISS1 insertion is dense in the region, except in two distinct genes, eqbA and SEQ_1258. The labels indicate the areas zoomed into in B and C of the figure. b. eqbE to SEQ_1247. ISS1 insertion is dense, except for in eqbA, the regulator of the equibactin locus. Equibactin aids the acquisition of iron, which if unregulated leads to excessive iron import and a slow growth phenotype. c. SEQ_1254 to SEQ_1262. ISS1 insertion is dense, except for in SEQ_1258, a putative antitoxin. ICESe2 encodes a putative toxin-antitoxin system which maintains the ICE in the bacterial genome. Both eqbA and SEQ_1258 were identifed as essential genes. Data is viewed in window size 9 for (a) and 3 for (b) and (c), with a maximum display value of 100 reads for ease of viewing. Data is viewed in Artemis [31]