Fig. 1

Fibroblast Response to LPS in Young versus Old Cultures. Interleukin-8 (a & c) and Interleukin-6 (b & d) protein production and gene expression were measured in young and old dermal fibroblasts (n = 6 per group) at various time points post LPS. Protein production is presented in pg/ml following 36 h of LPS stimulation. Gene expression was measured by RT-qPCR and is presented as the change in cycles to threshold (dCT) of gene expression at 0, 2 & 8 h post LPS in comparison to β-actin. Fold change gene expression (Old > Young) is presented in parentheses above each significant time point. All values are displayed as mean (+/− SEM). Significance was measured using a paired Student’s t test at each time point and ** = P < 0.01 *** = P < 0.001